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Covalent reinforcement of a fragile region in the dimeric enzyme thymidylate synthase...

Authors :
Gokhale, Rajesh S.
Agarwalla, Sanjay
Source :
Biochemistry. 06/04/96, Vol. 35 Issue 22, p7150. 9p. 1 Diagram, 14 Graphs.
Publication Year :
1996

Abstract

Presents a study where the covalent reinforcement of a fragile region in the dimeric enzyme thymidylate synthase stabilizes the protein against chaotrope-induced unfolding. Lactobacillus casei; Protein isolation; Fluorescence; Labeling of the active site cysteine; Circular dichroism.

Subjects

Subjects :
*ENZYMES
*DENATURATION of proteins

Details

Language :
English
ISSN :
00062960
Volume :
35
Issue :
22
Database :
Academic Search Index
Journal :
Biochemistry
Publication Type :
Academic Journal
Accession number :
9606300098
Full Text :
https://doi.org/10.1021/bi952890e