A ΔdinB mutation that sensitizes Escherichia coli to the lethal effects of UV- and X-radiation.

Highlights: [•] We describe Δ(dinB-yafN)883(::kan), a novel dinB allele, referred to as ΔdinB883, a deletion that sensitizes E. coli cells to UV irradiation. [•] This UV radiation sensitivity is most acute in the early logarithmic phase of culture growth. [•] This UV radiation sensitivity is completely dependent upon a functional umuDC operon. [•] Sequencing reveals ΔdinB883 retains the proximal 161 nucleotides, i.e., 54 amino acids, of the wild-type sequence. [•] The ΔdinB883 mutant is hypothesized to produce a peptide of 83 amino acids, DinB883, that compromises UmuDC function. [ABSTRACT FROM AUTHOR]