Purification and biochemical characterisation of glucoamylase from a newly isolated Aspergillus niger: Relation to starch processing.

Highlights: [•] The purified glucoamylase was a 57–62kDa polypeptide with pI 4.3–4.4. [•] Optimum temperature/pH, K m, V max and k cat of the enzyme were 70°C, 5, 0.33 (mgml−1), 0.095 (Uμg−1 min−1) and 158.3 (S−1), respectively. [•] Fe3+, Al3+ and Hg2+ inhibited, and Ag2+, Ca2+, Zn2+, Mg2+, Cd2+ ions and EDTA did not inhibit the enzyme considerably. [•] The purified enzyme may be useful for industrial starch processing. [ABSTRACT FROM AUTHOR]