Duration of the protective immune response after prime and booster vaccination of yearlings with a live modified cold-adapted viral vaccine against equine influenza.

Highlights: [•] We prepared novel live cold-adapted equine influenza viral vaccine. [•] The vaccine was completely safe for yearlings. [•] Single vaccination of yearlings inducing significant protection against the homologous virus at 12 months. [•] Double mode vaccination generated significant protection against the heterologous virus at 12 months. [•] This vaccine enables the differentiation of infected and vaccinated animals. [Copyright &y& Elsevier]