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Transfection of BmCPV genomic dsRNA in silkmoth-derived Bm5 cells: Stability and interactions with the core RNAi machinery.

Authors :
Swevers, Luc
Kolliopoulou, Anna
Li, Zheng
Daskalaki, Maria
Verret, Frederic
Kalantidis, Kriton
Smagghe, Guy
Sun, Jingchen
Source :
Journal of Insect Physiology. May2014, Vol. 64, p21-29. 9p.
Publication Year :
2014

Abstract

Highlights: [•] Long term stability of dsRNA in Bm5 cells after transfection. [•] Processing of BmCPV dsRNA by RNAi machinery. [•] Presence of BmCPV dsRNA does not interfere with gene silencing. [•] Transfection of BmCPV dsRNA genome in Bm5 cells does not generate functional virus. [Copyright &y& Elsevier]

Details

Language :
English
ISSN :
00221910
Volume :
64
Database :
Academic Search Index
Journal :
Journal of Insect Physiology
Publication Type :
Academic Journal
Accession number :
95828473
Full Text :
https://doi.org/10.1016/j.jinsphys.2014.03.002