MICROEXTRACTION IN PACKED SORBENT FOR ANALYSIS OF SULFONAMIDES IN POULTRY LITTER WASTEWATER SAMPLES BY LIQUID CHROMATOGRAPHY AND SPECTROPHOTOMETRIC DETECTION.

Rapid and sensitive analytical method for the simultaneous determination of sulfonamides (sulfacetamide, sulfadiazine, sulfathiazole, sulfamethazine, sulfamethoxypyridazine, and sulfamethoxazole) in poultry litter wastewater samples by microextraction packed sorbent (C8) and liquid chromatography with spectrophotometric detection (MEPS/LC) analysis. We evaluated how MEPS variables, such as the pH extraction, draw-eject cycles, ionic strength, and desorption conditions (solvent and solvent volume) affected extraction performance, to obtain a more efficient extraction process in the proposed method. The proposed MEPS/LC method presented linear range at concentrations from 5.0 ng mL−1(Limit of quantification - LOQ) to 200 ng mL−1, inter-day precision with coefficient of variation lower than 8%, correlation coefficient greater than 0.998; the extraction phase C8 was used. On the basis of analytical validation, the MEPS/LC methodology can be used to analyze sulfonamides in poultry litter wastewater samples. [ABSTRACT FROM PUBLISHER]