Back to Search Start Over

Construction of an engineered strain capable of degrading two isomeric nitrophenols via a sacB- and gfp-based markerless integration system.

Authors :
Hu, Fan
Jiang, Xuan
Zhang, Jun-Jie
Zhou, Ning-Yi
Source :
Applied Microbiology & Biotechnology. May2014, Vol. 98 Issue 10, p4749-4756. 8p.
Publication Year :
2014

Abstract

In this study, a gfp-based novel markerless allelic exchange integration system was developed. By employing gfp gene and sacB gene as counter-selectable markers, an ortho-nitrophenol degradation operon ( onpABC gene cluster) was successfully inserted into the chromosome of meta-nitrophenol utilizer Cupriavidus necator JMP134. Through two rounds of recombination, the engineered strain (strain JMP134-ONP) was directly selected from the plate by fluorescence screening and has the ability to degrade both ortho-nitrophenol and meta-nitrophenol, simultaneously. This relatively simple and efficient method can be used as an alternative strategy of allelic exchange insertion for the application of metabolic engineering in various bacterial strains, complementary to existing gene knock-in procedures. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
01757598
Volume :
98
Issue :
10
Database :
Academic Search Index
Journal :
Applied Microbiology & Biotechnology
Publication Type :
Academic Journal
Accession number :
95800400
Full Text :
https://doi.org/10.1007/s00253-014-5567-0