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Gerbil interleukin-18 and caspase-1: cloning, expression and characterization
- Source :
-
Gene . Mar2003, Vol. 307 Issue 1/2, p159. 8p. - Publication Year :
- 2003
-
Abstract
- We are reporting the molecular cloning of gerbil interleukin-18 (IL-18) and caspase-1. The cDNAs encoding the molecules were cloned by cross-species reverse transcriptase–polymerase chain reaction (RT–PCR) and 5′/3′ rapid amplification of cDNA ends (RACE). COS-7 cells transfected with plasmids encoding pro-IL-18 and caspase-1 precursor expressed the two proteins intracellularly. When the cells were lysed in the presence of dithiothreitol, caspase-1 precursor became active and converted pro-IL-18 into mature IL-18. A partially purified preparation of gerbil mature IL-18 was bioactive, as it stimulated the proliferation of gerbil spleen cells in a dose-dependent manner. [Copyright &y& Elsevier]
- Subjects :
- *BIOLOGICAL assay
*NUCLEOTIDE sequence
Subjects
Details
- Language :
- English
- ISSN :
- 03781119
- Volume :
- 307
- Issue :
- 1/2
- Database :
- Academic Search Index
- Journal :
- Gene
- Publication Type :
- Academic Journal
- Accession number :
- 9572915
- Full Text :
- https://doi.org/10.1016/S0378-1119(03)00457-8