Combining glycocluster synthesis with protein engineering: an approach to probe into the significance of linker length in a tandem-repeat-type lectin (galectin-4).

Highlights: [•] Synthesis of glycoclusters presenting lactose and 2′-fucosyl lactose on different scaffolds. [•] Engineering of galectin-4 mutants to probe structure–activity relationships with glycoclusters. [•] The impact of structural alterations by combining application of glycoclusters and mutants. [Copyright &y& Elsevier]