Inhibition of inflammatory response in LPS induced macrophages by 9-KOTE and 13-KOTE produced by biotransformation.

Highlights: [•] α-Linolenic acid (ALA) was biotransformed by Aspergillus niger into species with different degree of oxygenation. [•] The main biotransformation products were characterized by LC-MS/MS analyses. [•] Their anti-inflammatory potential was evaluated with NO and TNF-α quantification in LPS-stimulated RAW264.7 macrophages. [•] The isomeric ketoacids 9-KOTE and 13-KOTE were the most active. [•] Biotransformation was successfully employed for the production of potential analogs to lipid mediators. [Copyright &y& Elsevier]