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microRNA-146 up-regulation predicts the prognosis of non-small cell lung cancer by miRNA in situ hybridization.
- Source :
-
Experimental & Molecular Pathology . Apr2014, Vol. 96 Issue 2, p195-199. 5p. - Publication Year :
- 2014
-
Abstract
- Abstract: Non-small cell lung cancer (NSCLC) accounts for approximately 70% of all lung cancer-related deaths worldwide. Prognostic markers are essential for the early detection of lung cancer in patients. In this study, we first identified microRNA146 (miR-146) expression in cancer cell lines using miRNA in situ hybridization (MISH) and confirmed the accuracy of MISH using q-RT-PCR. In addition, two different systems, BCIP/NBT and ELF, were used to detect the signal for a comparative analysis of the specificity of MISH. Compared to the BCIP/NBT system, the ELF detection system was more effective for MISH. Furthermore we detected the expression of miR-146 in NSCLC tissues (43 cases) and normal tissues (32 cases). Based on our results, we can conclude that miR-146 is more highly expressed in cancer tissue than normal tissue (t-test, P<0.05) and that miR-146 can predict the prognosis of NSCLC by MISH. Our findings preliminary demonstrate that MISH can be applied as a molecular diagnostic tool to determine the expression and localization of miRNAs in cancer tissues and that miR-146, determined by MISH, predicts the prognosis of NSCLC patients. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 00144800
- Volume :
- 96
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Experimental & Molecular Pathology
- Publication Type :
- Academic Journal
- Accession number :
- 95462336
- Full Text :
- https://doi.org/10.1016/j.yexmp.2013.11.004