Determination of the average volumetric flow rate in supercritical fluid chromatography.

Highlights: [•] The average volumetric flow rate of a compressible mobile phase along a column is determined. [•] Estimates of the variations of pressure and temperature along the column are discussed. [•] This determination is based on knowledge of the mobile phase average density and mass flow rate. [•] It is key to understanding the retention mechanisms taking place inside the column. [•] It is validated by conversion of the retention times of nitrous oxide assumed to be non retained. [Copyright &y& Elsevier]