Back to Search Start Over

A procedure for setting up high-throughput nano-litre crystallization experiments. I. Protocol design and validation.

A procedure for setting up high-throughput nano-litre crystallization experiments. I. Protocol design and validation.

Authors :
Walter, T.S.
Diprose, J.
Brown, J.
Pickford, M.
Owens, R.J.
Stuart, D.I.
Harlos, K.
Source :
Journal of Applied Crystallography. Apr2003, Vol. 36 Issue 2, p308. 7p.
Publication Year :
2003

Abstract

A protocol for setting up nanolitre sitting-drop vapour-diffusion experiments is reported. The procedure uses standard crystallization screening kits and 96-well crystallization plates. Reservoir solutions are transferred from 96-deep-well blocks to crystallization plates in a single step with a Robbins-Hydra pipettor. Nanolitre droplets of protein as well as reservoir solution are dispensed by a Cartesian pipetting instrument. Experiments have been carried out to characterize the performance of this instrument. Adaptations to the Cartesian, which include an anti-evaporation cover plate, are described and tested. The protocol was designed for a high-throughput facility, but can be used in any standard crystallography laboratory. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00218898
Volume :
36
Issue :
2
Database :
Academic Search Index
Journal :
Journal of Applied Crystallography
Publication Type :
Academic Journal
Accession number :
9517389
Full Text :
https://doi.org/10.1107/S0021889803001997