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A procedure for setting up high-throughput nano-litre crystallization experiments. I. Protocol design and validation.
A procedure for setting up high-throughput nano-litre crystallization experiments. I. Protocol design and validation.
- Source :
-
Journal of Applied Crystallography . Apr2003, Vol. 36 Issue 2, p308. 7p. - Publication Year :
- 2003
-
Abstract
- A protocol for setting up nanolitre sitting-drop vapour-diffusion experiments is reported. The procedure uses standard crystallization screening kits and 96-well crystallization plates. Reservoir solutions are transferred from 96-deep-well blocks to crystallization plates in a single step with a Robbins-Hydra pipettor. Nanolitre droplets of protein as well as reservoir solution are dispensed by a Cartesian pipetting instrument. Experiments have been carried out to characterize the performance of this instrument. Adaptations to the Cartesian, which include an anti-evaporation cover plate, are described and tested. The protocol was designed for a high-throughput facility, but can be used in any standard crystallography laboratory. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CRYSTALLIZATION
*NANOTECHNOLOGY
*MICROPIPETTES
Subjects
Details
- Language :
- English
- ISSN :
- 00218898
- Volume :
- 36
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Journal of Applied Crystallography
- Publication Type :
- Academic Journal
- Accession number :
- 9517389
- Full Text :
- https://doi.org/10.1107/S0021889803001997