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Characteristics of Lactoferrin Receptor in Bovine Intestine: Higher Binding Activity to the Epithelium Overlying Peyer's Patches.
- Source :
-
Journal of Veterinary Medicine Series A . Apr2003, Vol. 50 Issue 3, p123-131. 9p. - Publication Year :
- 2003
-
Abstract
- Summary Several lines of evidence have recently demonstrated the occurrence of specific lactoferrin (Lf) receptors in different cells. We report here, for the first time, the characteristics of binding, and distribution of Lf receptors in the bovine intestinal tract with special emphasis on the epithelium overlying Peyer's patches (EOPP). Brush-border membrane vesicles (BBMV) were prepared from the mucosa of duodenum, jejunum, ileum, colon, EOPP in jejunum and EOPP in ileum. Receptor binding assays were carried out using 125 I-labelled bovine Lf. Specific and saturable Lf receptors were found in BBMV of all the intestinal segments examined. Non-linear regression and Scatchard plot analyses clearly revealed that EOPP had the highest binding maximal (Bmax ), and lowest in colon. The maximum dissociation constant (Kd ) 3.74 μ m was in the ileum. We found that bovine transferrin competed with Lf for the same binding site of receptors. In contrast, no binding of bovine serum albumin occurred. It was concluded that Lf receptors in the mucosal lining are attributable to mediate multifunctional activities of Lf in the gut, especially in the EOPP. [ABSTRACT FROM AUTHOR]
- Subjects :
- *LACTOFERRIN
*CATTLE
*INTESTINES
*EPITHELIUM
Subjects
Details
- Language :
- English
- ISSN :
- 0931184X
- Volume :
- 50
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Journal of Veterinary Medicine Series A
- Publication Type :
- Academic Journal
- Accession number :
- 9510585
- Full Text :
- https://doi.org/10.1046/j.1439-0442.2003.00512.x