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RVG-Peptide-Linked Trimethylated Chitosan for Deliveryof siRNA to the Brain.
- Source :
-
Biomacromolecules . Mar2014, Vol. 15 Issue 3, p1010-1018. 9p. - Publication Year :
- 2014
-
Abstract
- In this work, a peptide derived fromthe rabies virus glycoprotein(RVG) was linked to siRNA/trimethylated chitosan (TMC) complexes throughbifunctional PEG for efficient brain-targeted delivery of siRNA. Thephysiochemical properties of the complexes, such as siRNA complexingability, size and ζ potential, morphology, serum stability,and cytotoxicity, were investigated prior to studying the cellularuptake, in vitrogene silencing efficiency, and in vivobiodistribution. The RVG-peptide-linked siRNA/TMCâPEGcomplexes showed increased serum stability, negligible cytotoxicity,and higher cellular uptake than the unmodified siRNA/TMCâmPEGcomplexes in acetylcholine receptor positive Neuro2a cells. The potentknockdown of BACE1, a therapeutic target in Alzheimerâs disease,demonstrated the gene silencing efficiency. In vivoimaging analysis showed significant accumulation of Cy5âsiRNAin the isolated brain of mice injected with RVG-peptide-linked complexes.Therefore, the RVG-peptide-linked TMCâPEG developed in thisstudy can be used as a potential carrier for delivery of siRNA tothe brain. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15257797
- Volume :
- 15
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Biomacromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 94946104
- Full Text :
- https://doi.org/10.1021/bm401906p