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Effects of ascorbic acid, alpha-tocopherol, and glutathione on microspore embryogenesis in Brassica napus L.
- Source :
-
In Vitro Cellular & Developmental Biology Plant . Mar2014, Vol. 50 Issue 1, p26-35. 10p. - Publication Year :
- 2014
-
Abstract
- The impact of culture conditions and addition of antioxidants to media on microspore embryogenesis in rapeseed ( Brassica napus cv. 'PF704') was investigated. Different concentrations of ascorbic acid (0, 5, 10, 20, 50, 100, and 200 mg l) and alpha (α)-tocopherol (0, 5, 10, 20, 50, 100, and 200 mg l) were evaluated along with two temperature pretreatments (18 d at 30°C; 2 d at 32.5°C followed by 16 d at 30°C). In addition, combinations of reduced glutathione (0, 10, 50, and 100 mg l) and ascorbic acid (5 and 10 mg l) were tested. Microspore embryogenesis was significantly enhanced using 10 mg l ascorbic acid (334 embryos per Petri dish) compared with untreated cultures (184 embryos per Petri dish) at 30°C. α-Tocopherol (5 and 10 mg l) enhanced (312 and 314 embryos per Petri dish, respectively) microspore embryogenesis relative to untreated cultures (213 embryos per Petri dish) at 30°C, although there were no significant differences among cultures treated with 5-50 mg l α-tocopherol. When 50 mg l α-tocopherol was combined with 5 or 10 mg l ascorbic acid, embryogenesis was significantly enhanced (308 and 328 embryos per Petri dish, respectively) relative to other ascorbic acid levels. Moreover, 10 mg l of reduced glutathione and 5 mg l ascorbic acid enhanced microspore embryogenesis (335 embryos per Petri dish) compared to cultures without reduced glutathione (275 embryos per Petri dish). Microspore embryogenesis could be improved by adding ascorbic acid, α-tocopherol, and reduced glutathione when the appropriate combination and temperature pretreatment were selected. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10545476
- Volume :
- 50
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- In Vitro Cellular & Developmental Biology Plant
- Publication Type :
- Academic Journal
- Accession number :
- 94725055
- Full Text :
- https://doi.org/10.1007/s11627-013-9579-8