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RIPiT-Seq: A high-throughput approach for footprinting RNA:protein complexes.

Authors :
Singh, Guramrit
Ricci, Emiliano P.
Moore, Melissa J.
Source :
Methods. Feb2014, Vol. 65 Issue 3, p320-332. 13p.
Publication Year :
2014

Abstract

Highlights: [•] RIPiT-Seq is a novel RNP footprinting approach based on two sequential purifications. [•] Unveils transcriptome-wide binding sites of a single RBP or multi-subunit RNPs. [•] Distinguishes between footprints of RNPs with overlapping yet distinct composition. [•] Suitable for all RBPs irrespective of their RNA recognition mode. [ABSTRACT FROM AUTHOR]

Subjects

Subjects :
*NUCLEOTIDE sequence
*RNA-protein interactions
*CARRIER proteins
*NUCLEIC acid analysis
*BINDING sites
*CROSSLINKING of nucleic acids

Details

Language :
English
ISSN :
10462023
Volume :
65
Issue :
3
Database :
Academic Search Index
Journal :
Methods
Publication Type :
Academic Journal
Accession number :
94640134
Full Text :
https://doi.org/10.1016/j.ymeth.2013.09.013
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