Mass spectrometry-based quantification of the cellular response to methyl methanesulfonate treatment in human cells.

Highlights: [•] Spatial proteomics was used to monitor protein localization following MMS treatment. [•] MMS treatment affected the levels of many proteins in the chromatin-enriched fraction. [•] Proteins involved in chromatin organization were responsive to MMS treatment. [•] MMS treatment also affected components of the ubiquitin proteasome system. [•] Decreased phosphorylation of many nuclear proteins was observed after MMS treatment. [Copyright &y& Elsevier]