Elucidating Hydroxylation and Methylation Steps TailoringPiericidin A1 Biosynthesis.

The piericidin A1 (1) gene cluster was identifiedfrom the deep-sea derived Streptomycessp. SCSIO03032. Our in vivo and in vitro experiments verified PieE as a 4′-hydroxylaseand PieB2 as a 4′-O-methyltransferase, allowingthe elucidation of the post-PKS modification steps involved in 1biosynthesis. In addition, the shunt metabolite piericidinE1 (7) was identified as a novel analogue featuring aC-2/C-3 epoxy ring. [ABSTRACT FROM AUTHOR]