Simultaneous quantification of idelalisib, fludarabine and lenalidomide in rat plasma by using high-performance liquid chromatography coupled with heated electrospray ionization tandem mass spectrometry.

Highlights: [•] Highly selective method for the determination of idelalisib, fludarabine and lenalidomide in plasma. [•] This is the first LC-MS/MS-based method for simultaneous determination of idelalisib, fludarabine and lenalidomide in plasma. [•] Plasma sample clean-up involves a simple liquid–liquid extraction procedures. [•] The developed LC-MS/MS-based method was applied successfully for pharmacokinetic study. [ABSTRACT FROM AUTHOR]