Development of a minor groove binder assay for real-time PCR detection of porcine Sapelovirus.

Highlights: [•] A 5′ conjugated minor groove binder (MGB) probe real-time PCR assay was developed in this study for porcine sapelovirus (PSV) detection and quantitation. [•] The developed assay was capable of detecting about 103 copies/μl of standard template per reaction. [•] The coefficients of variation of intra- and inter-assay reproducibility were both lower than 2%. [ABSTRACT FROM AUTHOR]