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Preparation and validation of monoclonal antibody-based indirect competitive ELISA for detecting testosterone levels.
- Source :
-
Food & Agricultural Immunology . Jun2014, Vol. 25 Issue 2, p256-266. 11p. - Publication Year :
- 2014
-
Abstract
- This paper presents the generation of monoclonal antibodies (mAbs) against testosterone (TES) through cell fusion technology, and the development of a mAb-based indirect competitive enzyme-linked immunosorbent assay (icELISA) method to detect TES residue in bovine edible tissues. Under optimal conditions, this assay exhibited a working range of 0.04–19 ng/mL, with half-maximum inhibition (IC50) and limit of detection values of 0.27 and 0.02 ng/mL, respectively. After three sample pretreatment procedures were checked, a simple dilution method was adopted for further use. The stabilisation studies demonstrated that the icELISA kits can be stored for at least 180 to 240 days, at 4°C and −20°C, respectively. When applied to bovine samples, the data from icELISA and gas chromatography coupled to mass spectrometry showed excellent correlation (r2=0.9923 in muscle, 0.9884 in liver and 0.9957 in kidney). Therefore, this assay has the potential to be incorporated into a quantitative monitoring programme for the rapid screening of TES residue in foods. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09540105
- Volume :
- 25
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Food & Agricultural Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 94381082
- Full Text :
- https://doi.org/10.1080/09540105.2013.775221