Catechine biotransformation by tannase with sequential addition of substrate.

Highlights: [•] Tannase was used less than 1% tea extract as substrate because of substrate inhibition. [•] Tannase reaction was inhibited by the addition of 1% EGCG. [•] Sequential addition of substrate might solve the substrate inhibition of Tannase. [•] Sequential addition of substrate is appropriate process for the enrichment of active components. [ABSTRACT FROM AUTHOR]