Análise das possíveis Regiões Organizadoras Nucleolares e da atividade nucleolar em Triatoma melanocephala e T. lenti, importantes vetores da doença de Chagas.

Triatoma melanocephala and T. lenti are important vectors of Chagas disease. These cryptic species of triatomines are grouped in the subcomplex brasiliensis due only to morphological characters and geographical distribution. Cytogenetic studies are important to the classification of insects and can assist in creating a disease prevention plan. The aim of the present study was to determine nucleolar activity and nucleolus organizer regions (NORs) in these insects using the cytogenetic method of silver ion impregnation. T. melanocephala exhibited three active NORs in autosomes during prophase I. T. lenti exhibited two active NORs in autosomes during prophase I and metaphase I. Both species exhibit the persistent nucleolar material found in triatomines. The analysis of NORs in the present study revealed that T. lenti exhibited labeling similar to that found in T. tibiamaculata, which belongs to the subcomplex, whereas T. melanocephala shows no direct relationship with the subcomplex. [ABSTRACT FROM AUTHOR]