Determination of nitrofuran metabolites in shrimp by high performance liquid chromatography with fluorescence detection and liquid chromatography–tandem mass spectrometry using a new derivatization reagent.

Highlights: [•] A novel fluorescent derivatizing agent 2-hydroxy-1-naphthaldehyde (HN) is developed. [•] A new HPLC–FLD method for the determination of the derivatized metabolites of NF in shrimp is developed. [•] Sample cleanup is achieved using LLE without the adjustment of the pH and further SPE. [•] Separation is achieved on a YMC-Pack Polymer C18 column under alkaline conditions. [ABSTRACT FROM AUTHOR]