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Surface charge of bacteriorhodopsin detected with covalently bound pH indicators at selected...
- Source :
-
Biochemistry . 1/11/94, Vol. 33 Issue 1, p298. 9p. 1 Diagram, 1 Chart, 8 Graphs. - Publication Year :
- 1994
-
Abstract
- Presents a method that allows the detection of the surface charge density of bacteriorhodopsin at any selected protein surface site. Covalent binding of optical pH indicator fluorescein to the sulfhydryl groups of single cysteine residues; Calculation of the local surface charge density.
- Subjects :
- *BACTERIORHODOPSIN
*FLUORESCEIN
Subjects
Details
- Language :
- English
- ISSN :
- 00062960
- Volume :
- 33
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 9404191717
- Full Text :
- https://doi.org/10.1021/bi00167a039