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Self-assembled monolayer facilitates epithelial-mesenchymal interactions mimicking odontogenesis.
- Source :
-
Connective Tissue Research . Jan2014, Vol. 55 Issue 1, p26-33. 8p. - Publication Year :
- 2014
-
Abstract
- Cell-cell interactions are vital for embryonic organ development and normal function of differentiated cells and tissues. In this study we have developed a self-assembled monolayer-based co-culture system to study tooth morphogenesis. Specifically, we designed a 2-D microenvironment present in the dental tissue by creating a well-structured, laterally organized epithelial and mesenchymal cell co-culture system by patterning the cell-attachment substrate. Chemical modifications were used to develop tunable surface patterns to facilitate epithelial-mesenchymal interactions mimicking the developing tooth. Such a design promoted interactions between monolayer's of the 2 cell types and provided signaling cues that resulted in cellular differentiation and mineralized matrix formation. Gene expression analysis showed that these co-cultures mimicked in-vivo conditions than monolayer cultures of a single cell type. [ABSTRACT FROM AUTHOR]
- Subjects :
- *DENTIN
*EPITHELIAL cells
*MESENCHYMAL stem cells
*DENTAL matrices
*DENTITION
Subjects
Details
- Language :
- English
- ISSN :
- 03008207
- Volume :
- 55
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Connective Tissue Research
- Publication Type :
- Academic Journal
- Accession number :
- 93718830
- Full Text :
- https://doi.org/10.3109/03008207.2013.867335