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A validated HPLC method for determination of furazolidone and oxytetracycline in the presence of related substances.
- Source :
-
Veterinary Drug / Medicamentul Veterinar . 2013, Vol. 7 Issue 2, p48-54. 7p. - Publication Year :
- 2013
-
Abstract
- The objective of the current study was to develop a simple, precise, rapid and accurate reverse phase liquid chromatographic method for the quantitative determination on furazolidone, oxytetracycline and related substances in veterinary formulation. This formulation was submitted to accelerated degradation studies under acidic, alkaline and oxidative conditions, exposure to light and thermal stability. The separation of furazolidone, oxytetracycline and degradation products was achieved on BDS Hypersil C18 (250mmx4.6mm, i.d. 5 pm particle size) with gradient mobile phase containing methanol and 80 mM dipotassium phosphate pH 7,5 (20/80). The flow rate was 1.0 mL/min and detection was set at 254 nm, at 25 0C. The developed method was validated with respect to linearity, limits of detection and quantification, specificity, accuracy, and precision. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 18439527
- Volume :
- 7
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Veterinary Drug / Medicamentul Veterinar
- Publication Type :
- Academic Journal
- Accession number :
- 93611946