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CD56negCD16+ NK cells are activated mature NK cells with impaired effector function during HIV-1 infection.
- Source :
-
Retrovirology . 2013, Vol. 10 Issue 1, preceding p1-26. 27p. 1 Black and White Photograph, 2 Charts, 5 Graphs. - Publication Year :
- 2013
-
Abstract
- Background A subset of CD3negCD56negCD16+ Natural Killer (NK) cells is highly expanded during chronic HIV-1 infection. The role of this subset in HIV-1 pathogenesis remains unclear. The lack of NK cell lineage-specific markers has complicated the study of minor NK cell subpopulations. Results Using CD7 as an additional NK cell marker, we found that CD3negCD56negCD16+ cells are a heterogeneous population comprised of CD7+ NK cells and CD7neg non-classical myeloid cells. CD7+CD56negCD16+ NK cells are significantly expanded in HIV-1 infection. CD7+CD56negCD16+ NK cells are mature and express KIRs, the C-type lectin-like receptors NKG2A and NKG2C, and natural cytotoxicity receptors similar to CD7+CD56+CD16+ NK cells. CD7+CD56neg NK cells in healthy donors produced minimal IFNγ following K562 target cell or IL-12 plus IL-18 stimulation; however, they degranulated in response to K562 stimulation similar to CD7+CD56+ NK cells. HIV-1 infection resulted in reduced IFNγ secretion following K562 or cytokine stimulation by both NK cell subsets compared to healthy donors. Decreased granzyme B and perforin expression and increased expression of CD107a in the absence of stimulation, particularly in HIV-1-infected subjects, suggest that CD7+CD56negCD16+ NK cells may have recently engaged target cells. Furthermore, CD7+CD56negCD16+ NK cells have significantly increased expression of CD95, a marker of NK cell activation. Conclusions Taken together, CD7+CD56negCD16+ NK cells are activated, mature NK cells that may have recently engaged target cells. [ABSTRACT FROM AUTHOR]
- Subjects :
- *KILLER cells
*HIV infections
*BIOMARKERS
*PERFORINS
*CYTOKINES
Subjects
Details
- Language :
- English
- ISSN :
- 17424690
- Volume :
- 10
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Retrovirology
- Publication Type :
- Academic Journal
- Accession number :
- 93597631
- Full Text :
- https://doi.org/10.1186/1742-4690-10-158