Assessment The Viability of Mesenchymal Stem Cell Like Cells from Meningioam, Low Grade and High Grade Brain Tumor Samples after Treatment with Genistein.

Objective: Brain tumors are mostly developing tumors with low survival rate worldwide. Among different types of brain tumors, high grade gliomas are not curable while low grade gliomas are well differentiated tumors with better prognosis. Meningiomas are various sets of brain tumors arising from the meninges. Genistein has been recently introduced with anti-proliferative and anti-angiogenic potent effects in a variety of tumors. In this study the effects of Genistein on the proliferation rate and viability of meningioma, low grade and high grade glioma tumor cells was assessed. Materials and Methods: Brain tumor tissues obtained from patients diagnosed with different types of brain tumors were washed with PBS, cut into small pieces, digested with Collagenase type 1 and cultured in the tissue culture flask containing DMEM and 10% FBS. In passage 3 of culture, cells were treated with different concentrations of Genistein (0, 0.01, 1/250, 1/500 and 0.001 M) for 72 hours and then IC 50 was evaluated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Expression of Fas was determined using quantitative Real-Time PCR before and after treatment with Genistein. Results: Tumor cells have plastic adherent ability with mesenchymal like appearance and the ability to be cultured to 10 passages. High grade and low grade gliomas showed approximately 2-fold decrease in viability after treatment with Genistein (p value<0.05). Meningioma cells also showed 1.4-fold vitality decrease post treatment. Expression of Fas transcripts showed statistically significant higher expression in high grade glioma and meningioma samples after Genistein treatment compared to untreated cells (p value<0.05). Conclusion: Results of this study have important implications for the application of Genistein in different types of brain tumors as a therapeutic intervention in future [ABSTRACT FROM AUTHOR]