HGF mRNA Expression in Mesenchymal Stem Cell Like Cells from Glioma Tumor Tissue Samples Treated with Genistein.

Objective: Despite advances in surgical and adjuvant radiation therapy and chemotherapy strategies, malignant gliomas continue to be associated with poor prognosis in patients. To address suitable strategies, scientists pay close attention to factors that cause angiogenesis. Angiogenesis is a possible target in the treatment of human gliomas. Genistein has been previously introduced as an anti-angiogenic agent for different cancers. To evaluate the effect of Genistein on the angiogenic cascade, we investigated the expression of hepatocyte growth factor (HGF) transcripts in mesenchymal stem cell like cells from high grade and low grade brain tumors before and after treatment with Genistein. Materials and Methods: Brain tumor obtained from patient diagnoses with GBM and low grade glioma in sterile condition, wash with PBS, cut in to small pieces, digested with collagenase type I and culture in tissue culture flask contain MDEM 10% FBS. As cells arise to passage 3, they were treated with Genistein and then the HGF gene expression was determined by QRT-PCR in treated and untreated cells. Results: Genistein has a significant effect on both types of tumors after cells expose to compound compared to untreated cells. According to the results, Genistein has a remarkable effect on downregulating HGF transcripts where it showed 13×106- and 2-fold lower expression than untreated cells in high grade and low grade glioma cells, respectively. Conclusion: Our research showed that Genistein has a significant effect on downregulation of HGF. Consequently, Genistein have some important advantages for treatment of invasive brain tumors, however more investment cannot be ignore to make a suitable decision about effect of this compound on brain tumors [ABSTRACT FROM AUTHOR]