On-line sequential injection-capillary electrophoresis for near-real-time monitoring of extracellular lactate in cell culture flasks.

Highlights: [•] Automated sequential injection capillary electrophoresis for bioprocess monitoring. [•] Determination of lactate in media with intraday electrophoretic mobility RSD 0.07%. [•] Requires only 1.99mL of sample for 228 analyses (every 20min over 3 days). [•] Special sampling interface for sampling of cell-free media from adherent cell cultures. [•] Provides a unique insight in changes in metabolism and cell health over time. [ABSTRACT FROM AUTHOR]