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On-line determination of fluconazole in blood and dermal rat microdialysates by microbore high-performance liquid chromatography
- Source :
-
Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences . Apr2003, Vol. 787 Issue 2, p323. 9p. - Publication Year :
- 2003
-
Abstract
- To study the distribution of fluconazole in the dermis of the rat, on-line microdialysis using double-site sampling coupled with a microbore HPLC system was developed. The chromatographic conditions consisted of a mobile phase of 20 mM diammonium phosphate–acetonitrile (75:25, v/v, pH 7.0) pumped through a microbore C18 column at 40 μl/min. The eluent was monitored with UV detector with UZ flow cell (30 mm path length) at 210 nm. A microbore 10-port pneumatic valve fitted with two loops of 1 μl was used to collect and directly inject microdialysates from jugular and dermal probes. The retention time was 5.8 min for fluconazole and 10.1 min for its fluorinated analog, UK-54373 used as a retrodialysis marker. The assay was precise, with inter- and intra-assay relative standard deviation values of 0.64 and 0.71%, respectively, and with a good linearity (r=0.999) in the range of 0.15–20 μg/ml with only 1 μl injected onto the column. The LOD and LOQ values for fluconazole were 0.100 and 0.150 μg/ml, respectively. The applicability of the method was demonstrated by studying the disposition of fluconazole in blood and dermis following i.v. bolus at a dose of 10 mg/kg. [Copyright &y& Elsevier]
- Subjects :
- *MICRODIALYSIS
*LIQUID chromatography
Subjects
Details
- Language :
- English
- ISSN :
- 15700232
- Volume :
- 787
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 9287906
- Full Text :
- https://doi.org/10.1016/S1570-0232(02)00961-3