One-step column chromatographic extraction with gradient elution followed by automatic separation of volatiles, flavonoids and polysaccharides from Citrus grandis.

Highlights: [•] Citrus grandis has volatiles, flavonoids and polysaccharides hardly being extracted. [•] One-step CCE extraction of all bioactive substances with gradient elution. [•] Simple extraction and separation with minimum use of solvents and energy. [•] All substances had 98% extraction rates and 95% recovery rates after separation. [•] Can be used widely with greatly increased utilisation of natural resources. [ABSTRACT FROM AUTHOR]