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Mast cell degranulation promotes ischemia–reperfusion injury in rat liver.
- Source :
-
Journal of Surgical Research . Jan2014, Vol. 186 Issue 1, p170-178. 9p. - Publication Year :
- 2014
-
Abstract
- Abstract: Background: Mast cells (MCs) play a role in ischemia–reperfusion (I/R) injury in many organs. However, a recent study found that MCs are not involved in I/R injury in isolated rat livers that were perfused only for 1 h. The purpose of this study is to reevaluate the role of MCs in hepatic I/R injury in rat. Materials and methods: A warm hepatic I/R injury model of 1 h ischemia followed by 24 h of reperfusion was used. MC modulation was induced via cromolyn injection or a method called MC depletion using compound 48/80. The effects of MC modulation were evaluated by toluidine blue staining and assessment of mast cell tryptase in sera. The role of MCs in I/R injury was evaluated by hematoxylin and eosin staining graded by Suzuki criteria, alanine aminotransferase and aspartate aminotransferase levels in sera, and malondialdehyde levels in liver homogenates. Results: First, MC degranulation peaked after 2 h of reperfusion and liver damage peaked after approximately 6 h of reperfusion. Second, a method called MC depletion previously used in the skin with repeated injections of compound 48/80 worked similarly in the hepatic setting. Third, stabilization of MCs with cromolyn or depletion of MCs with compound 48/80 each decreased hepatic I/R injury. The most noticeable effects of cromolyn and compound 48/80 treatment were observed after approximately 6 h of reperfusion. Conclusions: MC degranulation promotes hepatic I/R injury in rats. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 00224804
- Volume :
- 186
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Journal of Surgical Research
- Publication Type :
- Academic Journal
- Accession number :
- 92863289
- Full Text :
- https://doi.org/10.1016/j.jss.2013.08.021