2D/3D Quantification of bone morphometric parameter changes using X-ray microtomograpphy with different pixel sizes.

Abstract: In recent years, bone quantification led to a deeper knowledge of the 3D microarchitecture. In this study the bone architecture of rats was investigated based on 2D/3D morphometric analysis using microcomputed tomography, aiming at determining the effect of the image acquisition pixel on the quality of some 2D/3D morphometric parameters, such as porosity and trabecular density. Six pairs of bone samples were used and the scans were carried out using high microcomputed tomography system, operating at three different pixel sizes of 33.3μm, 15.0μm and 9.5μm. The results showed 2D parameters values lower than those obtained in the 3D analysis, mainly for trabecular density, separation and thickness. [Copyright &y& Elsevier]