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Development of a new in vitro skin sensitization assay (Epidermal Sensitization Assay; EpiSensA) using reconstructed human epidermis.

Authors :
Saito, Kazutoshi
Nukada, Yuko
Takenouchi, Osamu
Miyazawa, Masaaki
Sakaguchi, Hitoshi
Nishiyama, Naohiro
Source :
Toxicology in Vitro. Dec2013, Vol. 27 Issue 8, p2213-2224. 12p.
Publication Year :
2013

Abstract

Highlights: [•] Gene expression profile was examined in RhE model. [•] Five cellular stress related genes were significantly upregulated by DNFB and OXA. [•] Predictive performance of ATF3 gene displayed 100% accuracy to animal testing. [•] Tested four pre-/pro-haptens were correctly predicted by ATF3 and DNAJB4 genes. [•] A RhE-based assay could detect skin sensitizing potential of chemicals. [Copyright &y& Elsevier]

Details

Language :
English
ISSN :
08872333
Volume :
27
Issue :
8
Database :
Academic Search Index
Journal :
Toxicology in Vitro
Publication Type :
Academic Journal
Accession number :
92501583
Full Text :
https://doi.org/10.1016/j.tiv.2013.08.007