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Effect of the phosphodiesterase 4 inhibitor, rolipram, on retinoic acid-increased alkaline phosphatase activity in the mouse fibroblastic C3H10T1/2 cell line
- Source :
-
Archives of Oral Biology . Jan2003, Vol. 48 Issue 1, p63. 5p. - Publication Year :
- 2003
-
Abstract
- We have evaluated effects of a phosphodiesterase (PDE) 4 inhibitor on retinoic acid-increased alkaline phosphatase activity in the mouse fibroblastic C3H10T1/2 clone 8 (10T1/2) cell line. 10T1/2 cells were cultured in minimum essential medium (MEM) and 10% fetal bovine serum with or without 1 μM retinoic acid and/or the PDE 4 inhibitor, rolipram, and harvested at specific intervals before measurement of alkaline phosphatase activity, cAMP production in response to parathyroid hormone, osteocalcin synthesis and expression, and phosphodiesterase activity. Retinoic acid-increased alkaline phosphatase activity, and slightly enhanced cAMP production in response to parathyroid hormone. However, it did not affect osteocalcin synthesis and expression. In the presence of retinoic acid, PDE 4 activity was not changed. A PDE 4 inhibitor, rolipram, and cAMP analog, 8-bromo-cAMP dramatically increased retinoic acid’s ability to induce alkaline phosphatase activity. This is the first report that PDE 4 may be involved in regulation of retinoic acid-increased alkaline phosphatase activity. [Copyright &y& Elsevier]
- Subjects :
- *PHOSPHODIESTERASES
*TRETINOIN
Subjects
Details
- Language :
- English
- ISSN :
- 00039969
- Volume :
- 48
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Archives of Oral Biology
- Publication Type :
- Academic Journal
- Accession number :
- 9189266
- Full Text :
- https://doi.org/10.1016/S0003-9969(02)00166-8