Reverse transfection in microchamber arrays for cell migration assays.

Abstract: A microdevice was designed to prevent cross-contamination of cells and reagents among reverse transfection (RTF) spots by using nanoliter-scale culture chambers. The forces generated by surface tension were used to seed cells into all the culture chambers simultaneously and separate them for following migration assays. Using the microdevice, bladder carcinoma cells were reverse-transfected and a migration-related gene was detected by tracing transfected cells. This approach may provide a platform for robust RTF assay systems. [Copyright &y& Elsevier]