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PI4K2β/AP-1-Based TGN-Endosomal Sorting Regulates Wnt Signaling.
- Source :
-
Current Biology . Nov2013, Vol. 23 Issue 21, p2185-2190. 6p. - Publication Year :
- 2013
-
Abstract
- Summary: Endosomal membrane traffic serves crucial roles in cell physiology, signaling, and development [1–4]. Sorting between endosomes and the trans-Golgi network (TGN) is regulated among other factors by the adaptor AP-1, an essential component of multicellular organisms [5]. Membrane recruitment of AP-1 requires phosphatidylinositol 4-phosphate [PI(4)P], though the precise mechanisms and PI4 kinase isozyme (or isozymes) involved in generation of this PI(4)P pool remain unclear [6, 7]. The Wnt pathway is a major developmental signaling cascade and depends on endosomal sorting in Wnt-sending cells [8–10]. Whether TGN/endosomal sorting modulates signaling downstream of Frizzled (Fz) receptors in Wnt-receiving cells is unknown. Here, we identify PI4-kinase type 2β (PI4K2β) as a regulator of TGN/endosomal sorting and Wnt signaling. PI4K2β and AP-1 interact directly and are required for efficient sorting between endosomes and the TGN. Zebrafish embryos depleted of PI4K2β or AP-1 lack pectoral fins due to defective Wnt signaling. Rescue experiments demonstrate requirements for PI4K2β-AP-1 complex formation and PI4K2β-mediated PI(4)P synthesis. Furthermore, PI4K2β binds to the Fz-associated component Dishevelled (Dvl) and regulates endosomal recycling of Fz receptors and Wnt target gene expression. These data reveal an evolutionarily conserved role for PI4K2β and AP-1 in coupling phosphoinositide metabolism to AP-1-mediated sorting and Wnt signaling. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 09609822
- Volume :
- 23
- Issue :
- 21
- Database :
- Academic Search Index
- Journal :
- Current Biology
- Publication Type :
- Academic Journal
- Accession number :
- 91847220
- Full Text :
- https://doi.org/10.1016/j.cub.2013.09.017