Recombinant glycoprotein G analog for determination of specific immunoglobulins to herpes simplex virus type 2 by ELISA.

Highlights: [•] The expression vector containing of the glycoprotein G HSV-2 (gG2) immunodominant regions was constructed. [•] The recombinant GST-gG2 fusion protein was accumulated mainly in soluble form in the Escherichia coli cells. [•] The affinity constant of the specific IgG to GST-gG2 protein was determined. [•] The target antigen was used to design the ELISA kit for detection of type-specific IgG against HSV-2 in human serum or plasma. [•] GST-gG2-based indirect ELISA kit has been recommended for clinical diagnosis of HSV-2 and epidemiological studies. [Copyright &y& Elsevier]