Simultaneous depletion of immunoglobulin G and albumin from human plasma using novel monolithic cryogel columns.

Highlights: [•] Simultaneous depletion of immunoglobulin G and albumin from human plasma. [•] Novel monolithic cryogel columns for co-depletion of high abundant proteins. [•] IgG was targeted via protein A meanwhile albumin was targeted via cibacron blue F3GA. [•] The plasma samples were assayed with SDS-PAGE to analyze the depletion efficiency. [•] Promising candidate for blood-related proteomics in not only lab scale but also preparative scale. [ABSTRACT FROM AUTHOR]