Liquid chromatography tandem mass spectrometry in study of the pharmacokinetics of six steroidal saponins in rats.

Highlights: [•] We developed and validated a novel quantification method using HPLC–MS/MS. [•] It is for the first time we simultaneous determined six main steroidal saponins in Paris polyphylla in vivo. [•] We report the pharmacokinetics parameters of steroidal saponins in rat for the first time. [•] We report the steroidal saponins have a long MRT and low concentration. [•] We introduced the Accelerated Solvent Extraction in sample preparation. [ABSTRACT FROM AUTHOR]