Novel LC–MS/MS method for analyzing imperialine in rat plasma: Development, validation, and application to pharmacokinetics.

Highlights: [•] We developed and validated a novel LC–MS/MS method for imperialine quantification. [•] The method debuted on pharmacokinetic study of imperialine in rats. [•] Pharmacokinetic study was done at low doses (mg/kg, i.v., 5; p.o., 1, 5 and 10). [•] Oral bioavailability of 3 p.o. groups was 31.2%, 53.6% and 47.4%, respectively. [Copyright &y& Elsevier]