Back to Search
Start Over
Use of single-layer centrifugation with Androcoll-C to enhance sperm quality in frozen-thawed dog semen.
- Source :
-
Theriogenology . Nov2013, Vol. 80 Issue 8, p955-962. 8p. - Publication Year :
- 2013
-
Abstract
- Abstract: The aim of this study was to investigate whether single-layer centrifugation (SLC) with Androcoll-C could select good quality spermatozoa, including those with specific motility patterns, from doses of frozen dog semen. Semen from five dogs was collected and cryopreserved following a standard protocol. After thawing, the semen samples were divided in two aliquots, one of which was used as a control and the other one processed by SLC. Assessment of sperm motility (assessed by computer-assisted semen analysis), morphology (Diff-Quick staining), viability (dual staining with propidium iodine/acridine orange), and acrosome integrity (dual staining with propidium iodine/isothiocyanate-labeled peanut [Arachis hypogaea] agglutinin) were performed on aliquots of fresh semen, frozen-thawed control samples, and frozen-thawed SLC-treated preparations. A multivariate clustering procedure separated 57,577 motile spermatozoa into three subpopulations (sP): sP1 consisted of poorly active and nonprogressive spermatozoa (48.8%), sP2 consisted of moderately slow but progressive spermatozoa (13.3%), and sP3 consisted of highly active and/or progressive spermatozoa (37.8%). SLC with Androcoll-C yielded sperm suspensions with improved motility, viability, and acrosome integrity (P < 0.01). The frozen-thawed SLC-treated samples were enriched in sP3, representing 38.5% of the sperm population. Likewise, sP2 was more frequently observed after SLC, but not significantly so. From these results, we concluded that for dog semen samples selected by SLC with Androcoll-C after thawing, the sperm quality parameters, including motility patterns, are better than in frozen-thawed control samples. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 0093691X
- Volume :
- 80
- Issue :
- 8
- Database :
- Academic Search Index
- Journal :
- Theriogenology
- Publication Type :
- Academic Journal
- Accession number :
- 90421412
- Full Text :
- https://doi.org/10.1016/j.theriogenology.2013.07.027