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A Novel Calcineurin-interacting Protein, CNP-3, Modulates Calcineurin Deficient Phenotypes in Caenorhabditis elegans.
- Source :
-
Molecules & Cells (Elsevier B.V) . 2008, Vol. 25 Issue 4, p566-571. 6p. - Publication Year :
- 2008
-
Abstract
- Calcineurin (Cn) is a calcium/calmodulin-dependent serine/threonine protein phosphatase that has diverse functions in different cell types and organisms. We screened proteins interacting with the C. elegans CnA homolog, TAX-6, by the yeast two-hybrid system. CNP-3 (Calcineurin interacting protein-3) is a novel protein that physically interacts with the catalytic domain of TAX-6. It is strongly expressed in the nuclei of intestine, hypodermis, dorsal uterine regions and spermatheca. Expression begins around the 60-cell stage and proceeds during all larval stages and the adult. To elucidate the biological function of cnp-3 we isolated a cnp-3 deletion mutant. Since CNP-3 binds CnA, we looked at factors associated with calcineurin loss-of-function mutants, such as brood size, body size, serotonin- and levamisole-mediated egg-laying behavior. The cnp-3(jh145) single mutant had no gross defects compared to wild-type animal. However, the phenotypes of the double mutants, tax-6(p675);cnp- 3(jh145) and cnb-1(jh103);cnp-3(jh145), were more severe in terms of brood size, body size and serotoninmediated egg-laying defects than tax-6(p675) and cnb- 1(jh103), respectively. These results suggest that dysfunction of cnp-3 enhances certain calcineurin loss-offunction phenotypes in C. elegans. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10168478
- Volume :
- 25
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Molecules & Cells (Elsevier B.V)
- Publication Type :
- Academic Journal
- Accession number :
- 90381267
- Full Text :
- https://doi.org/10.1016/s1016-8478(23)17619-9