Determination of Prostaglandin E1 in dog plasma using liquid chromatography–tandem mass spectrometry and its application to a pharmacokinetic study.

Highlights: [•] An LC–MS/MS was developed and validated for the determination of PGE1 in beagle dog plasma. [•] An LLOQ of 10pg/mL was achieved. [•] Indomethacin was added to plasma to avoid significant endogenous interfere. [•] A simple one-step sample extraction was used followed by a short cycle time of 3min. [•] The method was applied to a pharmacokinetic study in dogs at a clinical relevant dose. [ABSTRACT FROM AUTHOR]