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The platelet-endothelium interaction mediated by lectin-like oxidized low-density lipoprotein receptor-1 reduces the intracellular concentration of nitric oxide in endothelial cells
- Source :
-
Journal of the American College of Cardiology (JACC) . Feb2003, Vol. 41 Issue 3, p499-507. 9p. - Publication Year :
- 2003
-
Abstract
- : ObjectivesTo address the potential role of the endothelial lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) in the thrombotic system, in this study we first examined whether platelet interaction with LOX-1 generated reactive oxygen species (ROS) and superoxide (O2·−) and then investigated the relationship between the intracellular production of O2·− and the availability of nitric oxide (NO).: BackgroundOxidative inactivation of NO is regarded as an important cause of its decreased biologic activity which may favor platelet-dependent arterial thrombosis.: MethodsBovine aortic endothelial cells (BAECs) and Chinese hamster ovary-K1 cells stably expressing bovine LOX-1 (BLOX-1-CHO) were incubated at different times with human platelets. The ROS, O2·−, and NO were measured in cells by flow cytometry.: ResultsThe incubation of BAECs and BLOX-1-CHO cells with human platelets induced a sharp and dose-dependent increase in intracellular concentration of ROS and O2·− (p from <0.01 to <0.001). The increase in intracellular concentration of O2·− was followed by a dose-dependent reduction in basal and bradykinin-induced intracellular NO concentration (p from <0.01 to <0.001). The increase in O2·− and the reduction of NO were inhibited by the presence of vitamin C and anti-LOX-1 monoclonal antibody (p < 0.001).: ConclusionsThe results of this study show that one of the pathophysiologic consequences of platelet binding to LOX-1 may be the inactivation of NO through an increased cellular production of O2·−. [Copyright &y& Elsevier]
- Subjects :
- *ENDOTHELIUM
*LIPOPROTEINS
*NITRIC oxide analysis
*REACTIVE oxygen species
*ANIMAL experimentation
*BIOLOGICAL models
*BLOOD platelets
*CATTLE
*CELL receptors
*COMPARATIVE studies
*CYTOSOL
*FLOW cytometry
*HAMSTERS
*RESEARCH methodology
*MEDICAL cooperation
*OXIDIZING agents
*PEROXIDES
*RESEARCH
*THROMBOSIS
*EVALUATION research
*FREE radical scavengers
*IN vitro studies
*CELL physiology
Subjects
Details
- Language :
- English
- ISSN :
- 07351097
- Volume :
- 41
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Journal of the American College of Cardiology (JACC)
- Publication Type :
- Academic Journal
- Accession number :
- 9010567
- Full Text :
- https://doi.org/10.1016/S0735-1097(02)02811-5