Back to Search Start Over

Evaluation and Assessment of Biosafety Carotenoid-biofortified Rice (PAC): Progeny Stability Study for Four Generations.

Evaluation and Assessment of Biosafety Carotenoid-biofortified Rice (PAC): Progeny Stability Study for Four Generations.

Authors :
Sung-Dug Oh
Soo-Yun Park
Soo-In Sohn
Jae-Kwang Kim
Jong-Sug Park
Soon Ki Park
Hyun Suk Cho
Byung-Ohg Ahn
Kijong Lee
Source :
Korean Journal of Breeding Science. 2012, Vol. 44 Issue 4, p579-587. 9p.
Publication Year :
2012

Abstract

The carotenoid-biofortified (PAC) rice was developed and confirmed to synthesis carotenoids (zeaxanthin, α-carotene and β-carotene) in rice endosperm. For biosafety assessment of PAC rice, the stability of the insert genes in carotenoidbiofortified rice at genomic and protein expression level through different generations (T3~T6) was studied. Southern blot analysis confirmed that the multi-generation stability of the genes : PAC gene (CrtI and Psy), promoters (35S and Glb), terminators (NOS and PinII), selectable marker (bar) and MAR gene. It was also confirmed that the backbone DNA containing antibacterial gene (aadA) was not present in PAC rice. Immuno-strip assay (PAT) showed that the bar gene was stably expressed through generations. HPLC analysis confirmed that carotenoids were consistently detected through T3~T6 generations. These results will be filed to biosafety assessment document of PAC rice. [ABSTRACT FROM AUTHOR]

Details

Language :
Korean
ISSN :
02503360
Volume :
44
Issue :
4
Database :
Academic Search Index
Journal :
Korean Journal of Breeding Science
Publication Type :
Academic Journal
Accession number :
90037666
Full Text :
https://doi.org/10.9787/KJBS.2012.44.4.579