A fluorescence-based method for evaluating inositol 1,4,5-trisphosphate receptor ligands: Determination of subtype selectivity and partial agonist effects.

Authors :: Tanimura, Akihiko
Mochizuki, Tetsuya
Morita, Takao
Nezu, Akihiro
Tojyo, Yosuke
Arisawa, Mitsuhiro
Shuto, Satoshi
Source :: Journal of Biotechnology. Sep2013, Vol. 167 Issue 3, p248-254. 7p.
Publication Year :: 2013
Abstract: Highlights: [•] We developed a new method for evaluating properties of IP3R ligands using fluorescent biosensors. [•] This method revealed a diversity in subtype selectivity of adenophostin A and its analogs. [•] We also found a compound showing a partial agonistic effect on IP3R1. [Copyright &y& Elsevier]

Subjects :: *INOSITOL trisphosphate receptors
*LIGANDS (Biochemistry)
*LIGAND binding (Biochemistry)
*FLUORESCENCE resonance energy transfer
*ADENOPHOSTIN
*YELLOW fluorescent protein

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